CH2252 - Instrumental Methods of Analysis |
UNIT I FUNDAMENTALS OF SPECTRAL ANALYSIS |
ELECTROMAGNETIC RADIATION: Regions and properties, Various energy levels,
Interaction of photons with matter, absorbance, & transmittance and their relationship,
Permitted energy levels for the electrons of an atom and simple molecules, classification
of instrumental methods based on physical properties.; VISIBLE SPECTROSCOPY AND
COLORIMETRY: Beer-Lambert’s Law, Limitations, Deviations (Real, Chemical and
Instrumental deviations) Nesslerimetry, Duboscq colorimetry, Estimation of inorganic ions
such as Fe, Ni and Nitrite using Beer-Lambert’s Law. UV-VISIBLE AND IR SPECTROSCOPY: Instrumentation (Source, Optical parts and Detectors) - Various
electronic transitions in organic and inorganic compounds effected by UV, Visible and
infra red radiations. Woodward-Fischer rules for the calculation of absorption maxima
(dienes and carbonyl compounds). Effects of auxochromes and effects of conjugation on
the absorption maxima, Multicomponent analysis - Photometric titration |
UNIT II ELECTROMETRIC METHODS |
Conductometric Titrations: Instrumentation-Types-Advantages-Application;
Potentiometric Titrations: Instrumentation-Types-Advantages-Application; Measurement
of pH: Instrumentation-Applications; Ion selective electrodes: Electrode setup-
Applications.; Amperometric titrations: Principle-instrumentation- Application |
UNIT IIIX-RAY DIFFRACTION & THERMAL ANALYSIS METHODS |
XRD: Introduction, Mosley's law, Different emission and diffraction methods, various Xray
detectors.
Thermogravimetic Analysis (TGA): Instrumentation, factors affecting the shapes of
thermograms, applications, thermograms of some important compounds (CuSO4, 5H2O,
CaC2O42H2O etc).
Differential thermal analysis (DTA) & Differential Scanning Calorimetry (DSC): Principle,
Instrumentation and applications of DSC and DTA, differences between DSC and DTA. |
UNIT IVIMPORTANT SPECTROSCOPIC METHODS OF ANALYSIS |
Atomic Absorption Spectroscopy (AAS): Principle, Instrumentation, Interference and
Applications.
Flame Photometry and Inductively coupled Plasma Atomic Emission spectroscopy(ICPAES): Principle, Instrumentation and Applications. Polarimetry: Principle, Instrumentation and
Applications.
Refractometry: Principle, Instrumentation and Applications. Nephlometry/Turbidimetry:
Principle, Instrumentation and Applications. |
UNIT VCHROMATOGRAPHIC METHODS |
Chromatographic methods - Types (column, Thin layer, paper, Gas, High performance
liquid Chromatographic methods) – principle- separation technique - separation of
organic compounds by column and thin layer, Amino acids and mixture of Cu, Co & Ni
by Paper, estimation of organic compounds by GC and HPLC.
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Text Book |
1. Skoog D.A. and West D.M., " Fundamentals of Analytical Chemistry ", Saunderscollege
Publishing, 1982.
2. Willard, H.H., Merritt. I.I., Dean J.a., and Settle, F.A., " Instrumental methods of
analysis ", Sixth edition, CBS publishers, 1986. |
References |
1. A.I.Vogel., "Qualitative Inorganic analysis ", V.Edition, Prentice-Hall of India (P)
Ltd., New Delhi, 1991.
2. S harma, B.K., " Instrumental Methods of Analysis ", Goel publishing House,
1995.
3. Parikh V.M., " Absorption spectroscopy of organic molecules ", Addison -
Wesley Publishing Company, 1974. |
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