1. Urine physical and chemical examination (protein, reducing substances, ketones,
bilirubin and blood)
2. Hematoxylin and eosin staining.
3. Study of parts of compound microscope
4. Histopathological slides of benign and malignant tumours.
5. Manual tissue processing and section cutting (demonstration)
6. Simple stain.
7. Gram stain.
8. AFB stain.
9. Slides of malyarial parasites, micro filaria and leishmania donovani.
10. Haematology slides of anemia and leukemia
11. Bleeding time and clotting time.
12. Study of bone marrow charts
Lab Requirement:
1) Test tubes, Test tube racks, Bunsen burner(gas),(Benedict’s solution, Acetic acid,
Sulphosalicylic acid), centrifuge, microscope.
2) Slides, cover slips,H & E stains
3) Microscope
4) Beakers,graded alcohols, acetone,Lmoulds(or maling bodies), paradigm wax, water
bath, microtone for section cutting,slides,cover slips,hotair oven,refrigerator to store
chemical and ice.
5) whanman filter paper for bleeding time Test tubes.
6) Simple Stain test
SIMPLE STAIN, Glass Slide
Reagents – Methylene blue, Crystal Violet, Cabal Iuschin
7) Gram Stain test
SIMPLE GRAM STAIN
Reagents – Crystal Violet, Gram’s Iodine, 95% ethyl alcohol Safranin
Urine bacterial count/ml exceeding 100,000(10s) denotes urinary tract infection.
Normal : 0-100 ml
Glass Slide
8) AFB Strain test
ACID FAST STAIN (ZIEHL – NEELSON METHOD)
Reagent – Carbal Iushin, Acid Alcohol, Methylene blue, Glass slide
9) Bone marrow charts