BM2205 - Biochemistry |
UNIT I |
Introduction to biochemistry – Biomolecules, structure of water & its importance –
Important noncovalent forces – Hydrogen bonds, electrostatic, hydrophobic &
vanderwaals forces – Acid, base & buffers – pH, Henderson Hasselbalch equation.
Biological buffers and their significance – Principle of viscosity – surface tension ,
adsorption, diffusion, osmosis & their applications in biological systems. |
UNIT II |
Classification, structure & properties of carbohydrates – mono, di , oligo &
polysaccharides. Classification, structure & properties of amino acids & proteins.
Classification, structure & properties of Lipids – Simple lipids , Phospholipids , glycolipids & steroids . Transport of lipids: Lipoproteins Structure & functions of nucleic acids –
Nucleosides , nucleotides – Cyclic AMP , cyclic GMP , ATP , GTP – DNA & RNA |
UNIT III |
Classification of Enzymes, Chemical nature, Active Site, Specificity of Enzyme catalyzed
reactions, Regulation : Feedback , Allosteric , Covalent modification , Hormonal
regulation, co-enzymes. Assay of enzymes, enzymes in clinical diagnosis of diseases.
Introduction to Metabolism: Carbohydrate metabolism, Glycolysis
Lipid metabolism : fatty acid, beta oxidation , ketogenesis and cholesterol metabolism.
TCA cycle : Structure of biological membranes, electron transport & Oxidative
phosphorylation. |
UNIT IV |
Liver function and liver function tests, Kidney function and kidney function tests , normal& abnormal constituents of urine and their clinical significance. General characteristics of
hormones. Structure , functions & disorders of thyroid , parathyroid , pituitary ,adrenal
and pancreatic hormones. Hormones as chemical messengers: General assay of
hormones – Bio assay , chemical assay & immuno assays. |
UNIT V |
Analytical techniques: Principle and applications of electrophoresis – PAGE , SDS
PAGE , Isoelectric focusing , Two Dimensional Electrophoresis. Chromatography:
Principle of adsorption & partition chromatography, Size exclusion , Ion exchange &
affinity chromatography. Spectro photometry, fluorimetry, flame photometry, manometry
, microcalorimetry , electrochemical methods, biosensors , automation in clinical
laboratory , use of radio isotopes in biochemistry. |
Text Book |
1. Harper’s review of biochemistry By David. W. Martin, Peter. A. Mayes , Victor. W.
Rodwell . LANGE medical publications, 2003.
2. Practical Biochemistry – Principles & Techniques,Keith Wilson & John Walker. Oxford
university press. |
References |
1. Understanding Enzymes By Trevor palmer. Published by Ellis Horwood LTD.
2. Biochemistry Lippincott’s Illustrated Reviews By Pamela.C.Champe & Richard.
A.Harvey. Lippincott-Raven publishers, 3rd edition, 2006. |
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