1. Laboratory safety and sterilization techniques
2. Microscopic methods in the identification of microorganisms
3. Preparation of culture media nutrient broth and nutrient agar
4. Culturing of microorganisms in broth and in plates (pour plates, streak plates, isolation and preservation of bacterial cultures)
5. Staining techniques grams and differential
6. Quantitation of microorganisms.
7. Effect of disinfectants on microbial flora
8. Isolation and identification of microorganisms from different sources soil, water and milk
9. Antibiotic sensitivity assay
10. Growth curve observation and growth characteristics of bacteria and yeast.
11. Effect of different parameters on bacterial growth (ph, temperature & UV irradiation)
REFERENCE
Micro Biology : Laboratory Theory and applications, M.J. Heboffee aw BE Pierce Morten Publishing House, 2006.