1. Introduction to principles of sterile techniques and cell propagation.
2. Principles of microscopy, phase contrast and fluorescent microscopy.
3. Identification of given plant, animal and bacterial cells and their components by microscopy,
4. GRAM’S Staining,
5. Leishman Staining,
6. Thin Layer Chromatography,
7. Giemsa Staining,
8. Separation of Peripheral Blood Mononuclear Cells from blood,
9. Osmosis and Tonicity,
10. Tryphan Blue Assay,
11. Staining for different stages of mitosis in AlliumCepa (Onion).
REFERENCE
Reference Books
1. Laboratory Investigations in Cell and Molecular Biology, Allen Bregman Wiley, 2001.